The Keck NMR Center for Structural Biology
This facility houses three NMR spectrometers located on the ground floor of the Centers for Molecular Medicine (CMM). Access can be granted after training and checkouts given by the facility staff. Detailed user guides are available to assist in the use of the instruments.
The two spectrometers listed below constitute the instruments maintained as part of the Center. The instruments are made available to students and faculty of Stony Brook University on a scheduled basis (see access and use policies). Fees are applied in the facility for all users. Training is required to obtain access to the instruments.
Avance 600
Three channel system equipped with kW amplifiers on all channels for advanced solid state NMR experiments. Wide bore magnet.
- 2.5mm H/F/X probe
- low frequency channel: 15N-13C
- high frequency channel: 19F-1H
- spinning speed: 35 kHz
- 4mm H/F/X probe
- low frequency channel: 15N-13C
- high frequency channel: 19F-1H
- spinning speed: 15 kHz
- 4mm H/XY probe
- X channel from 15N-13C or 13C-31P
- Y channel from 15N-17O
- spinning speed: 15 kHz
- 4mm H/X/Y probe
- for 13C-31P, 13C/17O, 13C/15N REDOR experiments
- spinning speed: 15 kHz
- static triple resonance probe
- tuning range: 1H/13C/15N + 31P
- VT range: -100°C/+150°C
Avance 700
Four channel system with three X nuclei amplifiers (300 and 500W) and one high frequency amplifier, three axis pulse field gradient accessory. Standard bore magnet.
- 5mm inverse broadband probe, with Z-gradient coil
- Low frequency channel: 109Ag-31P
- VT range: -120°C to +80°C
- 5mm inverse triple resonance probe, with X, Y and Z- gradient coils
- 1H/13C/15N
- VT range: -20°C to +80°C
- 2.5mm H-F/X probe
- low frequency channel: 13C-31P
- high frequence channel: 19F-1H
- spinning speed: 35kHz
Access and Use Policies
The Center for Structural Biology allows access to the Keck NMR Center to all Stony Brook University campus users. Other academic and industrial users are given access with the approval of the facility director. All users must go through proper training and checkouts before attempting to use any of the equipment. Unauthorized use of the equipment may result in access limitations, or more drastic measures in the case of equipment damage.
On Campus Users
A student who wishes to operate instruments in the Center must first obtain approval from his or her research supervisor. He or she will then be instructed in proper operation of the instrument and approved as an operator by the facility staff.
All postdoctoral, graduate and undergraduate students involved in research projects and/or supporting graduate research within the Center for Structural Biology must discuss access with the facility director. Experimental protocols and preliminary results on lower field instruments will be required. These students will be trained accordingly by the facility staff.
Students requiring access to the instruments for solids applications are expected to be able to perform standard experiments like CP-MAS or single pulse experiments on their own. Help will be provided for advanced experiments that are already coded. Direct supervision by the facility staff is required for experiments that are not yet programmed.
Off-Campus Academic Users
Scientists from other universities can use the facility upon approval of the facility director. Access can be granted when high fields or advanced solids experiments are required. For routine liquid work, these users should use the NMR facility in the Department of Chemistry.
Other Users and Service Work
The Keck NMR Center for Structural Biology can assist local industry and small regional schools with the occasional special research problem involving high field solution or solid state NMR.
Direct access to the equipment by external users is limited and requests should be discussed with the facility director.
Signup Procedures
To sign up for instrument time on any instrument in the NMR Center, first send an email request to Martine Ziliox (martine.ziliox@sunysb.edu) specifying:
- the type of experiment to be run
- the time block desired
- the temperature
- if assistance is needed
- if there are deadlines for the project
The smallest time interval that can be reserved is 1 hour. Email requests
will be reviewed on a weekly basis.
Users are not permitted to reassign
any portion of their reserved time to another user. If you think you may finish
early and another user is interested in using the remaining time, you must make
prior arrangement with the facility staff. If several members of a group are
planning to use the time, notify Martine Ziliox of that possibility when the
reservation is made.
The 1st of the month is reserved for maintenance. No reservation will be accepted for those days unless it has been arranged directly with Martine Ziliox. Nitrogen fills will be performed every Friday. Please keep this in mind when planning your measurement time.
Rates
Usage is be computed from the time a user is logged in to the NMR program.
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